Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles

Sean Wei Xiang Ong; Pei Yun Hon; Sharon Syn Hui Wee; Jonathan Wei Zhong Chia; Shehara Mendis; Ezlyn Izharuddin; Ray Junhao Lin; Po Ying Chia; Rees Chin Swee Sim; Mark I-Cheng Chen; Angela Chow; Joanne Yoong; David Chien Lye; Christine B Teng; Paul Anantharajah Tambyah; Ritu Banerjee; Robin Patel; Partha Pratim De; Shawn Vasoo

doi:10.1093/cid/ciab848

Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles

Clin Infect Dis. 2022 May 30;74(10):1850-1854. doi: 10.1093/cid/ciab848.

Authors

Sean Wei Xiang Ong^{1

2}, Pei Yun Hon¹, Sharon Syn Hui Wee³, Jonathan Wei Zhong Chia⁴, Shehara Mendis⁴, Ezlyn Izharuddin¹, Ray Junhao Lin^{1

2}, Po Ying Chia^{1

2

5}, Rees Chin Swee Sim⁴, Mark I-Cheng Chen^{1

6}, Angela Chow^{5

6

7}, Joanne Yoong⁶, David Chien Lye^{1

2

5

8}, Christine B Teng⁹, Paul Anantharajah Tambyah^{8

10}, Ritu Banerjee¹¹, Robin Patel¹², Partha Pratim De⁴, Shawn Vasoo^{1

2

5}

Affiliations

¹ National Centre for Infectious Diseases, Singapore.
² Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore.
³ Clinical Research and Innovation Office, Tan Tock Seng Hospital, Singapore.
⁴ Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore.
⁵ Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.
⁶ Saw Swee Hock School of Public Health, National University of Singapore, Singapore.
⁷ Department of Clinical Epidemiology, Office of Clinical Epidemiology, Analytics, and Knowledge (OCEAN), Tan Tock Seng Hospital, Singapore.
⁸ Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
⁹ Department of Pharmacy, National University of Singapore, Singapore.
¹⁰ Division of Infectious Disease, National University Hospital, Singapore.
¹¹ Division of Pediatric Infectious Diseases, Vanderbilt University, Nashville, Tennessee, USA.
¹² Divisions of Clinical Microbiology and Infectious Diseases, Mayo Clinic, Rochester, Minnesota, USA.

PMID: 34554228
DOI: 10.1093/cid/ciab848

Abstract

We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum β-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.

Trial registration: ClinicalTrials.gov NCT02743585.

Keywords: ESBL; antimicrobial resistance; carbapenemase; diagnostics; gram-negative bacteria; rapid test kits.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Bacteremia* / diagnosis
Bacterial Proteins
Blood Culture
Gram-Negative Bacteria / genetics
Humans
Microbial Sensitivity Tests
Multiplex Polymerase Chain Reaction*
beta-Lactamases / genetics

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase

Associated data

ClinicalTrials.gov/NCT02743585