Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord

Methods Mol Biol. 2022:2389:103-110. doi: 10.1007/978-1-0716-1783-0_10.


We demonstrated the presence of neural stem cells and/or progenitor cells in the adult human spinal cord. This chapter provides materials and methods to harvest high-quality samples of thoracolumbar, lumbar, and sacral adult human spinal cord and human dorsal root ganglia isolated from brain-dead patients who had agreed before passing to donate their bodies to science for therapeutic and scientific advances. The methods to culture precursor cells from the adult human spinal cord are also described.

Keywords: Adult human spinal cord; Organ donation; Precursor cells; Stem cells.

MeSH terms

  • Adult
  • Cell Culture Techniques
  • Cell Separation
  • Ganglia, Spinal
  • Humans
  • Neural Stem Cells*
  • Spinal Cord*