A longitudinal sampling study of transcriptomic and epigenetic profiles in patients with thrombocytopenia syndrome

Nat Commun. 2021 Sep 24;12(1):5629. doi: 10.1038/s41467-021-25804-z.

Abstract

Severe fever with thrombocytopenia syndrome (SFTS) is a novel tick-borne infectious disease caused by a new type of SFTS virus (SFTSV). Here, a longitudinal sampling study is conducted to explore the differences in transcript levels after SFTSV infection, and to characterize the transcriptomic and epigenetic profiles of hospitalized patients. The results reveal significant changes in the mRNA expression of certain genes from onset to recovery. Moreover, m6A-seq reveals that certain genes related with immune regulation may be regulated by m6A. Besides the routine tests such as platelet counts, serum ALT and AST levels testing, distinct changes in myocardial enzymes, coagulation function, and inflammation are well correlated with the clinical data and sequencing data, suggesting that clinical practitioners should monitor the above indicators to track disease progression and guide personalized treatment. In this study, the transcript changes and RNA modification may lend a fresh perspective to our understanding of the SFTSV and play a significant role in the discovery of drugs for effective treatment of this disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Alanine Transaminase / blood
  • Antiviral Agents / therapeutic use
  • Aspartate Aminotransferases / blood
  • Creatinine / blood
  • Epigenesis, Genetic*
  • Epigenomics / methods*
  • Female
  • Gene Expression Profiling / methods*
  • Hospitalization / statistics & numerical data
  • Humans
  • Longitudinal Studies
  • Male
  • Middle Aged
  • Phlebovirus / drug effects
  • Phlebovirus / physiology
  • RNA-Seq / methods
  • Sampling Studies
  • Severe Fever with Thrombocytopenia Syndrome / drug therapy
  • Severe Fever with Thrombocytopenia Syndrome / genetics*
  • Severe Fever with Thrombocytopenia Syndrome / virology
  • Transcriptome*

Substances

  • Antiviral Agents
  • Creatinine
  • Aspartate Aminotransferases
  • Alanine Transaminase

Supplementary concepts

  • SFTS phlebovirus