Association of miR-146a and miR196a2 genotype with susceptibility to idiopathic recurrent pregnancy loss in Iranian women: A case-control study

Emad Babakhanzadeh; Hamid Danaei; Mohammad Abedinzadeh; Hamid Reza Ashrafzadeh; Nasrin Ghasemi

doi:10.18502/ijrm.v19i8.9620

Association of miR-146a and miR196a2 genotype with susceptibility to idiopathic recurrent pregnancy loss in Iranian women: A case-control study

Int J Reprod Biomed. 2021 Sep 9;19(8):725-732. doi: 10.18502/ijrm.v19i8.9620. eCollection 2021 Aug.

Authors

Emad Babakhanzadeh^{1

2}, Hamid Danaei³, Mohammad Abedinzadeh³, Hamid Reza Ashrafzadeh³, Nasrin Ghasemi^{1

3}

Affiliations

¹ Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
² Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
³ Abortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Abstract

Background: Recurrent pregnancy loss (RPL) is the most common complaint of pregnancy in females with a prevalence of 5%. Numerous documents have shown that single nucleotide polymorphisms are able to change miRNA transcription and/or maturation, which may alter the incidence of disorders such as RPL.

Objective: To assess the relationship of miR-146aC $>$ G (rs2910164) and miR-196a2T $>$ C (rs11614913) with RPL susceptibility in Iranian women.

Materials and methods: Blood samples were collected from 214 women who had experienced at least two consecutive spontaneous miscarriages (case) and 147 normal individuals without a history of miscarriage (control). MiR-146aC $>$ G and miR-196a2T $>$ C genotypes were evaluated via the restriction fragment length polymorphism technique.

Results: The genotypes incidence did not show a significant difference in pre-miR-146aC $>$ G polymorphism CC vs CG + GG (p = 0.854; OR = 0.933; 95% CI) and CC + CG vs GG (p = 0.282; OR = 1.454; 95% CI). Also, no significant difference was observed between pre-miR-196a2T $>$ C polymorphism TT vs TC + CC (p = 0.862; OR = 0.938; 95% CI) and TT + TC vs CC and (p = 0.291; OR = 1.462; 95% CI) in both the case and control groups.

Conclusion: The results showed that although the distribution of miR-146aC $>$ G and miR-196a2T $>$ C was different between the unknown RPL and control groups, these variances were not statistically significant.

Keywords: Polymorphism; RFLP.; miR-146a; miR196a2; RPL.