Transforming growth factor type beta regulation of actin mRNA

J Cell Physiol. 1986 Apr;127(1):83-8. doi: 10.1002/jcp.1041270111.

Abstract

Stimulation of quiescent cultures of AKR-2B cells with transforming growth factor type beta (TGF beta) resulted in a transitory increase in actin cytoplasmic poly(A) + RNA. Levels of actin mRNA peaked approximately 4-8 hours subsequent to TGF beta addition and approached basal levels by 24 hours. The accumulation of actin transcripts was dose dependent and insensitive to inhibitors of protein synthesis; 1-3 ng/ml TGF beta induced maximal actin gene expression. Actin isotype-specific probes demonstrated that both beta- and gamma-cytoplasmic actins are induced by TGF beta.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actin Cytoskeleton / physiology
  • Actins / biosynthesis
  • Actins / genetics*
  • Animals
  • Cell Line
  • Cycloheximide / pharmacology
  • Dose-Response Relationship, Drug
  • Growth Substances / pharmacology*
  • Kinetics
  • Mice
  • Peptides / pharmacology*
  • Protein Biosynthesis / drug effects
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / genetics
  • Transcription, Genetic / drug effects
  • Transforming Growth Factors

Substances

  • Actins
  • Growth Substances
  • Peptides
  • RNA, Messenger
  • Transforming Growth Factors
  • Cycloheximide