Effects of a BMI1008 mixture on postoperative pain in a rat model of incisional pain

PLoS One. 2021 Sep 27;16(9):e0257267. doi: 10.1371/journal.pone.0257267. eCollection 2021.

Abstract

Background: The purpose of this study was to evaluate the analgesic effect of BMI1008 (a new drug containing lidocaine, methylene blue, dexamethasone and vitamin B complex) and to investigate the analgesic effect of lidocaine and BMI-L (other components of BMI1008 except lidocaine) at different concentrations in a rat model of incisional pain.

Methods: Male Sprague-Dawley rats (250-300 g) were used for the incisional pain model simulating postoperative pain. After the operation, normal saline, various concentrations of BMI1008, lidocaine with a fixed concentration of BMI-L, and BMI-L with a fixed concentration of lidocaine were injected at the incision site. The preventive analgesic effect was evaluated using BMI1008 administered 30 min before and immediately after the operation. In addition, BMI1008 was compared with positive controls using intraperitoneal ketorolac 30 mg/kg and fentanyl 0.5 μg/kg. The mechanical withdrawal threshold was measured with a von Frey filament.

Results: The analgesic effect according to the concentration of BMI1008, lidocaine with a fixed concentration of BMI-L, and BMI-L with a fixed concentration of lidocaine showed a concentration-dependent response and statistically significant difference among the groups (P <0.001, P <0.001, and P <0.001, respectively). The analgesic effect according to the time point of administration (before and after the operation) showed no evidence of a statistically significant difference between the groups (P = 0.170). Compared with the positive control groups, the results showed a statistically significant difference between the groups (P = 0.024).

Conclusion: BMI1008 showed its analgesic effect in a rat model of incisional pain in a concentration-dependent manner. Moreover, BMI-L showed an additive effect on the analgesic effect of lidocaine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analgesia / methods
  • Analgesics / administration & dosage*
  • Animals
  • Behavior, Animal
  • Dexamethasone / administration & dosage*
  • Dose-Response Relationship, Drug
  • Lidocaine / administration & dosage*
  • Male
  • Methylene Blue / administration & dosage*
  • Pain Management / methods*
  • Pain, Postoperative / prevention & control*
  • Pain, Postoperative / therapy
  • Rats
  • Rats, Sprague-Dawley
  • Surgical Wound
  • Vitamin B Complex / administration & dosage*

Substances

  • Analgesics
  • Vitamin B Complex
  • Dexamethasone
  • Lidocaine
  • Methylene Blue

Grants and funding

HK: BMI Korea supported BMI 1008 materials and research grants for this research. No role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. GJC: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology [Grant No. NRF-2020R1C1C1011263]. No role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.