Characterization of mitogenomes from four Mucorales species and insights into pathogenicity

Guanzhao Liang; Meijie Zhang; Wenqi Xu; Xiaowen Wang; Hailin Zheng; Huan Mei; Weida Liu

doi:10.1111/myc.13374

Characterization of mitogenomes from four Mucorales species and insights into pathogenicity

Mycoses. 2022 Jan;65(1):45-56. doi: 10.1111/myc.13374. Epub 2021 Oct 19.

Authors

Guanzhao Liang^{1

2

3}, Meijie Zhang^{1

2

3}, Wenqi Xu^{1

2

3}, Xiaowen Wang⁴, Hailin Zheng^{1

2

3}, Huan Mei^{1

2

3}, Weida Liu^{1

2

3

5}

Affiliations

¹ Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, China.
² CAMS Collection Center of Pathogen Microorganisms-D (CAMS-CCPM-D）, Nanjing, China.
³ Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, China.
⁴ Shanghai BIOZERON Biotechnology Co., Ltd., Shanghai, China.
⁵ Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China.

PMID: 34570921
DOI: 10.1111/myc.13374

Abstract

Background: Mucorales, as one major order of Zygomycetes fungi, can infect human beings and cause serious consequence. We have noticed the pathogenicity of Mucorales is closely related to energy metabolism, while mitochondria play the role of energy factories in almost all biological activities.

Methods: Virulence of M irregularis, M hiemalis, L corymbifera and R arrhizus were verified in Galleria mellonella larvae, as well as mitochondrial gene copies analysed with RT-qPCR. Mitogenomes of the four Mucorales species were sequenced based on illumina NovaSeq technology to study their characteristic features and functional regions.

Results: Variant virulence of M irregularis, M hiemalis, L corymbifera and R arrhizu were verified by clinical retrospective data and our G mellonella infection models, also copies of mitochondrial genes indicated the significant associations with pathogenicity. A total of 274.18 clean reads were generated to be assembled; the complete mitogenomes of the four Mucorales species were obtained with totally different length. After the genomes annotated and compared, M irregularis was found more similar with M hiemalis than those of L corymbifera and R arrhizus, especially the small (rrns) and large (rrnl) subunits of mitochondrial ribosomal RNA (rRNA) genes. The GC content, ncRNAs and the distribution of the SNPs and InDels were also compared, and the GC content rate of fungi seems to be related to the fungal thermal adaptability. In addition, linear mitogenomes of these four Mucorales showed diverse arrangements of orf genes and directionality of some conserved gene elements.

Conclusion: This study uncovered the pathogenicity variances among the four Mucorales species and the relationship between their mitogenomic features and clinical pathogenicity. Further studies like spatial structure of mitochondrial genomes and the comprehensive analysis of transcription regulation are needed.

Keywords: Lichtheimia corymbifera; Mucor hiemalis; Mucor irregularis; Rhizopus arrhizus; mitochondrial genome; pathogenicity.

MeSH terms

Genome, Fungal*
Genome, Mitochondrial*
Humans
Mucorales* / genetics
Mucorales* / pathogenicity
Mucormycosis
Virulence / genetics

Grants and funding

2020-PT310-005/2020 Project of Base Platform Construction