Searching for Biological Function of the Mysterious PA2504 Protein from Pseudomonas aeruginosa

Int J Mol Sci. 2021 Sep 11;22(18):9833. doi: 10.3390/ijms22189833.


For nearly half of the proteome of an important pathogen, Pseudomonas aeruginosa, the function has not yet been recognised. Here, we characterise one such mysterious protein PA2504, originally isolated by us as a sole partner of the RppH RNA hydrolase involved in transcription regulation of multiple genes. This study aims at elucidating details of PA2504 function and discussing its implications for bacterial biology. We show that PA2504 forms homodimers and is evenly distributed in the cytoplasm of bacterial cells. Molecular modelling identified the presence of a Tudor-like domain in PA2504. Transcriptomic analysis of a ΔPA2504 mutant showed that 42 transcripts, mainly coding for proteins involved in sulphur metabolism, were affected by the lack of PA2504. In vivo crosslinking of cellular proteins in the exponential and stationary phase of growth revealed several polypeptides that bound to PA2504 exclusively in the stationary phase. Mass spectrometry analysis identified them as the 30S ribosomal protein S4, the translation elongation factor TufA, and the global response regulator GacA. These results indicate that PA2504 may function as a tether for several important cellular factors.

Keywords: Nudix; PA2504; Pseudomonas aeruginosa; RNA-Seq; RppH; TUDOR domain; crosslink in vivo; sulphur metabolism.

MeSH terms

  • Bacterial Proteins* / chemistry
  • Bacterial Proteins* / genetics
  • Bacterial Proteins* / metabolism
  • Gene Deletion
  • Gene Expression Profiling
  • Models, Molecular*
  • Protein Domains
  • Protein Multimerization*
  • Pseudomonas aeruginosa* / chemistry
  • Pseudomonas aeruginosa* / genetics
  • Pseudomonas aeruginosa* / metabolism
  • Transcription, Genetic*


  • Bacterial Proteins