Antimicrobial, Antioxidant and Antiproliferative Secondary Metabolites from Inonotus nidus-pici

Molecules. 2021 Sep 7;26(18):5453. doi: 10.3390/molecules26185453.


Inonotus nidus-pici is a sterile conk which produces macrofungus, a neglected Central-Eastern European relative of the prized Inonotus obliquus, also known as chaga. Investigation of the methanol extract of the poroid fungus I. nidus-pici resulted in the isolation of citropremide (1), 3,4-dihydroxybenzalacetone (2) , lanosterol (3), ergost-6,8,22-trien-3β-ol (4), and ergosterol peroxide (5). The structures of fungal compounds were determined on the basis of one- and two-dimensional NMR and MS spectroscopic analysis. Compounds 1-2 and 4-5 were evaluated for their antioxidant and antimicrobial properties against several bacterial and fungal strains. 3,4-dihydroxybenzalacetone (2) and ergost-6,8,22-trien-3β-ol (4) demonstrated moderate antimicrobial activity, while the former possessed notable antioxidant activity in DPPH assay. The antiproliferative examinations performed on three human cancer (MES-SA, MES-SA/Dx5, A431) cell lines demonstrated that compounds 4 and 5 have notable cytotoxic activity with IC values in micromolar range. The current study represents the first report on the chemical profile of I. nidus-pici, providing a comprehensive study on the isolation and structure determination of bioactive secondary metabolites of this macrofungus.

Keywords: Hymenochaetaceae; antimicrobial; antiproliferative; ceramide; chaga; doxorubicin resistant; sclerotium; steroids.

MeSH terms

  • Agaricales
  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Antineoplastic Agents
  • Antioxidants*
  • Cell Line, Tumor
  • Fruiting Bodies, Fungal
  • Humans
  • Inonotus
  • Lanosterol*


  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Antineoplastic Agents
  • Antioxidants
  • Lanosterol