Establishment of TUSMi009-A, an induced pluripotent stem cell (iPSC) line from a 24-year-old Chinese Han patient with gliocytoma

Stem Cell Res. 2021 Oct:56:102546. doi: 10.1016/j.scr.2021.102546. Epub 2021 Sep 20.

Abstract

Glioblastoma is the most common malignant primary brain tumor with poor prognosis and low long-term survival rate. Here we described the production of an iPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 24-year-old female gliocytoma patient. The PBMCs were reprogrammed by the non-integrating Sendai Virus with human OKSM (OCT3/4, SOX2, KLF4, and c-MYC) transcription factors. The generated iPSCs were positive for pluripotent stem cell markers demonstrated by immunocytochemistry. Besides, they displayed a normal karyotype and had the potential to differentiate spontaneously three germ layers in vitro. Our model might be useful in studying the pathogenesis of gliocytoma patients.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cell Differentiation
  • China
  • Female
  • Germ Layers
  • Glioblastoma* / genetics
  • Humans
  • Induced Pluripotent Stem Cells*
  • Kruppel-Like Factor 4
  • Leukocytes, Mononuclear
  • Sendai virus
  • Young Adult

Substances

  • KLF4 protein, human
  • Kruppel-Like Factor 4