Abstract
Bacillus subtilis can form structurally complex biofilms on solid or liquid surfaces, which requires expression of genes for matrix production. The transcription of these genes is activated by regulatory protein RemA, which binds to poorly conserved, repetitive DNA regions but lacks obvious DNA-binding motifs or domains. Here, we present the structure of the RemA homologue from Geobacillus thermodenitrificans, showing a unique octameric ring with the potential to form a 16-meric superstructure. These results, together with further biochemical and in vivo characterization of B. subtilis RemA, suggests that the protein can wrap DNA around its ring-like structure through a LytTR-related domain.
© 2021. The Author(s).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus subtilis / physiology
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification
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Bacterial Proteins / metabolism*
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Bacterial Proteins / ultrastructure
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Biofilms / growth & development*
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Crystallography, X-Ray
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DNA, Bacterial / metabolism*
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Gene Expression Regulation, Bacterial
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Geobacillus / physiology*
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Models, Genetic
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Mutagenesis, Site-Directed
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Protein Interaction Domains and Motifs / genetics
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Protein Multimerization / genetics
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Recombinant Proteins / ultrastructure
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Regulatory Sequences, Nucleic Acid
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Transcription Factors / genetics
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Transcription Factors / isolation & purification
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Transcription Factors / metabolism*
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Transcription Factors / ultrastructure
Substances
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Bacterial Proteins
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DNA, Bacterial
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Recombinant Proteins
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Transcription Factors
Supplementary concepts
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Geobacillus thermodenitrificans