A multiplex PCR assay for the simultaneous detection of five potexviruses infecting cactus plants using dual-priming oligonucleotides (DPOs) primers

Chung Hwa Park; Eun Gyeong Song; Ki Hyun Ryu

doi:10.1016/j.jviromet.2021.114280

A multiplex PCR assay for the simultaneous detection of five potexviruses infecting cactus plants using dual-priming oligonucleotides (DPOs) primers

J Virol Methods. 2021 Dec:298:114280. doi: 10.1016/j.jviromet.2021.114280. Epub 2021 Sep 27.

Authors

Chung Hwa Park¹, Eun Gyeong Song¹, Ki Hyun Ryu²

Affiliations

¹ Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Republic of Korea.
² Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Republic of Korea. Electronic address: ryu@swu.ac.kr.

PMID: 34592336
DOI: 10.1016/j.jviromet.2021.114280

Abstract

Five potexviruses, namely, cactus virus X (CVX), opuntia virus X, pitaya virus X (PiVX), schlumbergera virus X (SchVX) and zygocactus virus X (ZyVX), have been reported in cactus plants. In this report, a multiplex RT-PCR assay, based on specific dual-priming oligonucleotide (DPO) primers, was developed to detect these five viruses simultaneously in field samples. Using 18 field plants comprising 16 cactus species, these viruses were detected among nine of the 18 plants, including the simultaneous detection of CVX, PiVX, SchVX and ZyVX co-infecting an Aporocactus flagelliformis and a Notocactus leninghausii f. cristatus plant. The multiplex PCR assay was thus applied successfully in the field plants and it would be useful in the diagnosis of viral infections in cactus plants.

Keywords: Cactus virus X; Multiplex PCR; Opuntia virus X; Pitaya virus X; Schlumbergera virus X; Zygocactus virus X.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cactaceae*
DNA Primers / genetics
Multiplex Polymerase Chain Reaction
Oligonucleotides
Potexvirus*
Sensitivity and Specificity

Substances

DNA Primers
Oligonucleotides