Early replication and expression of oocyte-type 5S RNA genes in a Xenopus somatic cell line carrying a translocation

Proc Natl Acad Sci U S A. 1986 Jul;83(14):5150-4. doi: 10.1073/pnas.83.14.5150.

Abstract

In Xenopus somatic cells, the somatic-type 5S RNA genes replicate early in S phase, bind the transcription factor TFIIIA, and are expressed; in contrast, the late replicating oocyte-type genes do not bind TFIIIA and are transcriptionally inactive. These facts support a model in which the order of replication of the somatic-type versus the oocyte-type 5S genes causes their differential expression in somatic cells due to sequestration of TFIIIA by the early-replicating somatic genes. Here we provide further evidence for the model by showing that in one Xenopus cell line in which some oocyte-type 5S genes are translocated, some oocyte-type 5S genes replicate early and are expressed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Division*
  • Cell Line
  • Female
  • Gene Expression Regulation*
  • Interphase
  • Models, Genetic*
  • Oocytes
  • RNA, Ribosomal / biosynthesis*
  • RNA, Ribosomal / genetics
  • Transcription Factor TFIIIA
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics
  • Translocation, Genetic
  • Xenopus laevis / genetics
  • Xenopus laevis / metabolism

Substances

  • RNA, Ribosomal
  • Transcription Factor TFIIIA
  • Transcription Factors