Triton X-100 supplementation regulates growth and secondary metabolite biosynthesis during in-depth extractive fermentation of Monascus purpureus

Pengxin Lu; Anqi Wu; Song Zhang; Jie Bai; Ting Guo; Qinlu Lin; Jun Liu

doi:10.1016/j.jbiotec.2021.09.018

Triton X-100 supplementation regulates growth and secondary metabolite biosynthesis during in-depth extractive fermentation of Monascus purpureus

J Biotechnol. 2021 Nov 20:341:137-145. doi: 10.1016/j.jbiotec.2021.09.018. Epub 2021 Oct 1.

Authors

Pengxin Lu¹, Anqi Wu¹, Song Zhang¹, Jie Bai¹, Ting Guo¹, Qinlu Lin¹, Jun Liu²

Affiliations

¹ National Engineering Laboratory for Deep Process of Rice and Byproducts, Hunan Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Key Laboratory of Processed Food for Special Medical Purpose, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 41004, China.
² National Engineering Laboratory for Deep Process of Rice and Byproducts, Hunan Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Key Laboratory of Processed Food for Special Medical Purpose, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 41004, China. Electronic address: liujundandy@csuft.edu.cn.

PMID: 34601020
DOI: 10.1016/j.jbiotec.2021.09.018

Abstract

Extractive fermentation has been proven to be efficient in enhancing the secretion and production of secondary metabolites in submerged fermentation by Monascus spp., owing to increased cell membrane permeability and resolved product inhibition. In this study, we investigated the regulation effect of Triton X-100 on cell growth and secondary metabolite biosynthesis in submerged fermentation of M. purpureus DK. The results show that the maximum monascus pigments (MPs), citrinin (CIT) production, and specific growth rate are 136.86 U/mL, 4.57 mg/L, and 0.04 h^-1, respectively, when 3 g/L of Triton X-100 is supplemented after fermentation for 10 d, and the extracellular MPs and CIT increased by 127.48% and 288.57%, respectively. RT-qPCR shows that the expression levels of MPs and CIT biosynthesis gene clusters are significantly upregulated, whereas those of glycolysis, tricarboxylic acid cycle, respiratory chains, and ATP synthase are downregulated. This study provides a vital strategy for extractive fermentation under extreme environmental conditions for further enhancing MP production.

Keywords: Cell growth; Citrinin; Extractive fermentation; Monascus pigments; Triton X-100.

MeSH terms

Citrinin*
Dietary Supplements
Fermentation
Monascus* / metabolism
Octoxynol
Pigments, Biological / metabolism

Substances

Pigments, Biological
Citrinin
Octoxynol