Chitosan-coated Selenium nanoparticles enhance the efficiency of stem cells in the neuroprotection of streptozotocin-induced neurotoxicity in male rats

Sara Soleimani Asl; Iraj Amiri; Alireza Samzadeh-Kermani; Roghayeh Abbasalipourkabir; Bahareh Gholamigeravand; Siamak Shahidi

doi:10.1016/j.biocel.2021.106089

Chitosan-coated Selenium nanoparticles enhance the efficiency of stem cells in the neuroprotection of streptozotocin-induced neurotoxicity in male rats

Int J Biochem Cell Biol. 2021 Dec:141:106089. doi: 10.1016/j.biocel.2021.106089. Epub 2021 Oct 1.

Authors

Sara Soleimani Asl¹, Iraj Amiri¹, Alireza Samzadeh-Kermani², Roghayeh Abbasalipourkabir³, Bahareh Gholamigeravand⁴, Siamak Shahidi⁵

Affiliations

¹ Endometrium and Endometriosis Research Centre, Hamadan University of Medical Sciences, Hamadan, Iran; Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
² Department of Chemistry, Faculty of Science, University of Zabol, Zabol, Iran.
³ Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
⁴ Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
⁵ Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address: shahidi@umsha.ac.ir.

PMID: 34601090
DOI: 10.1016/j.biocel.2021.106089

Abstract

Alzheimer's disease (AD) is one of the common neurodegenerative diseases characterized by memory impairment. The protective effects of stem cell-based therapy have been reported in AD. In this study, it was assumed that Chitosan-coated Selenium nanoparticles (ChSeNPs) increase the efficiency of stem cells in the attenuation of neurotoxicity in the rat AD model. The AD model was induced using Streptozotocin (STZ) and treated by the adipose-derived mesenchymal stem cells (AMSCs) and SeNPs/ChSeNPs (0.4 mg/kg). Passive avoidance learning and recognition memory were assessed using shuttle box and novel object recognition tasks. The amyloid-beta deposition, the injected cells' homing and survival, antioxidant capacity, and BDNF concentration were evaluated using the histological, biochemical, and ELISA methods. The results showed that the combined administration of ChSeNPs and AMSCs is more effective in increasing the step-through latency and discrimination index than administering SeNPs and stem cells. Combined therapy caused a significant increase in antioxidant capacity that ChSeNPs was more effective than SeNPs, while AMSCs beside SeNPs had a greater effect on BDNF levels compared to conventional treatment of nanoparticles or AMSCs alone. Ultimately, the homing and survival of the transplanted AMSCs were greater in the group that received both stem cells and ChSeNPs. Taken together, it seems that the administration of ChSeNPs enhances the efficiency of transplanted stem cells in decreasing the neurotoxicity induced by STZ through an increase in the antioxidant capacity.

Keywords: AMSCs; Alzheimer's disease; Chitosan-coated Selenium nanoparticles; Neuroprotection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Male
Nanoparticles
Neuroprotection
Rats
Selenium*
Stem Cells
Streptozocin*

Substances

Streptozocin
Selenium