Lactobacillus paracasei L9 improves colitis by expanding butyrate-producing bacteria that inhibit the IL-6/STAT3 signaling pathway

Food Funct. 2021 Nov 1;12(21):10700-10713. doi: 10.1039/d1fo02077c.

Abstract

Inflammatory bowel disease (IBD) is a chronic intestinal inflammation that is currently incurable. Increasing evidence indicates that supplementation with probiotics could improve the symptoms of IBD. It is scientifically significant to identify novel and valid strains for treating IBD. It has been reported that the probiotic Lactobacillus paracasei L9 (L9), which is identified from the gut of healthy centenarians, can modulate host immunity and plays an anti-allergic role. Here, we demonstrated that L9 alleviates the pathological phenotypes of experimental colitis by expanding the abundance of butyrate-producing bacteria. Oral administration of sodium butyrate in experimental colitis recapitulates the L9 anti-inflammatory phenotypes. Mechanistically, sodium butyrate ameliorated the inflammatory responses by inhibiting the IL-6/STAT3 signaling pathway in colitis. Overall, these findings demonstrated that L9 alleviates the DSS-induced colitis development by enhancing the abundance of butyrate-producing bacterial strains that produce butyrate to suppress the IL-6/STAT3 signaling pathway, providing new insight into a promising therapeutic target for the remission of IBD.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage
  • Anti-Inflammatory Agents / pharmacology
  • Butyrates
  • Butyric Acid / administration & dosage
  • Butyric Acid / pharmacology
  • Colitis / chemically induced*
  • Colitis / therapy*
  • Dextran Sulfate / toxicity
  • Female
  • Gene Expression Regulation / drug effects
  • Histamine Antagonists / administration & dosage
  • Histamine Antagonists / pharmacology
  • Inflammation / drug therapy
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism*
  • Lacticaseibacillus paracasei*
  • Mice
  • Mice, Inbred C57BL
  • Probiotics / therapeutic use*
  • Random Allocation
  • STAT3 Transcription Factor / genetics
  • STAT3 Transcription Factor / metabolism*

Substances

  • Anti-Inflammatory Agents
  • Butyrates
  • Histamine Antagonists
  • Interleukin-6
  • STAT3 Transcription Factor
  • Stat3 protein, mouse
  • interleukin-6, mouse
  • Butyric Acid
  • Dextran Sulfate