Background: Graph metrics of structural brain networks demonstrate to be a powerful tool for investigating brain topology at a large scale. However, the variability of the results related to applying different magnetic resonance acquisition schemes and tractography reconstruction techniques is not fully characterized. Materials and Methods: The present work aims to evaluate the influence of different combinations of diffusion acquisition schemes (single and multishell), diffusion models (tensor and spherical deconvolution), and tractography reconstruction approaches (deterministic and probabilistic) on the reproducibility of graph metrics derived from structural connectome on test/retest (TRT) data released by the Human Connectome Project. From each implemented experimental setup, both global and local graph metrics were evaluated and their reproducibility was estimated by the intraclass correlation coefficient (ICC). Moreover, the percentage relative standard deviation (pRSD) from the ICC values of local graph metrics was calculated to quantify how much the reproducibility varied across nodes within each experimental setup. Results: The presented results show that different combinations of diffusion acquisition schemes, diffusion models, and tractography algorithms can strongly affect the reproducibility of global and local graph metrics. The combination of constrained spherical deconvolution (CSD) and deterministic tractography gave generally high reproducibility (ICCs >0.75) and lowest pRSD for the considered graph metrics, meanwhile probabilistic CSD with a high b-value returned the highest reproducibility. Notably, the introduction of streamline selection filters on CSD can substantially affect the reproducibility. Discussion: This work demonstrates that the TRT reproducibility of graph metrics is generally high but can vary substantially with different combinations of acquisition and reconstruction schemes. Impact statement This work demonstrates the influence of different diffusion acquisition schemes, diffusion models, and tractography reconstruction approaches on the reproducibility of graph metrics derived from structural connectome. The presented findings impact on the choice of both acquisition protocol and processing pipeline for topological analyses to produce reproducible measurements for brain network studies.
Keywords: brain topology; graph metrics; reproducibility; structural connectome; test/retest; tractography.