A finger-driven disposable micro-platform based on isothermal amplification for the application of multiplexed and point-of-care diagnosis of tuberculosis

Zhiying Wang; Yang Wang; Long Lin; Tao Wu; Zhenzhen Zhao; Binwu Ying; Lingqian Chang

doi:10.1016/j.bios.2021.113663

A finger-driven disposable micro-platform based on isothermal amplification for the application of multiplexed and point-of-care diagnosis of tuberculosis

Biosens Bioelectron. 2022 Jan 1:195:113663. doi: 10.1016/j.bios.2021.113663. Epub 2021 Sep 22.

Authors

Zhiying Wang¹, Yang Wang², Long Lin¹, Tao Wu³, Zhenzhen Zhao⁴, Binwu Ying⁵, Lingqian Chang⁶

Affiliations

¹ Beijing Advanced Innovation Center for Biomedical Engineering, Key Laboratory for Biomechanics and Mechanobiology, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China.
² Beijing Advanced Innovation Center for Biomedical Engineering, Key Laboratory for Biomechanics and Mechanobiology, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China. Electronic address: w_yang89@163.com.
³ Department of Laboratory Medicine, Med+X Center for Manufacturing, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China; Department of Clinical Laboratory Medicine, People's Hospital of Ningxia Hui Autonomous Region (First Affiliated Hospital of Northwest Minzu University), Yinchuan 750002, China.
⁴ Department of Laboratory Medicine, Med+X Center for Manufacturing, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China.
⁵ Department of Laboratory Medicine, Med+X Center for Manufacturing, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China. Electronic address: yingbinwu@scu.edu.cn.
⁶ Beijing Advanced Innovation Center for Biomedical Engineering, Key Laboratory for Biomechanics and Mechanobiology, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China; School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Medical University, Hefei 230032, China. Electronic address: lingqianchang@buaa.edu.cn.

PMID: 34610534
DOI: 10.1016/j.bios.2021.113663

Abstract

Tuberculosis (TB) remains the high-risk infectious pathogen that caused global pandemic and high mortality, particularly in the areas lack in health resources. Clinical TB screening and diagnosis are so far mainly conducted on limited types of commercial platforms, which are expensive and require skilled personnel. In this work, we introduced a low-cost and portable finger-driven microfluidic chip (named Fd-MC) based on recombinase polymerase amplification (RPA) for rapid on-site detection of TB. After injection of the pre-treated sample solution, the pre-packaged buffer was driven by the pressure generated by the finger-actuated operation to accomplish sequential processes of diagnosis in a fully isolated microchannel. An in-situ fluorescence strategy based on FAM-probe was implemented for on-chip results read-out though a hand-held UV lamp. Hence, the Fd-MC proved unique advantageous for avoiding the risk of infection or environmental contamination. In addition, the Fd-MC was designed for multiplexed detection, which is able to not only identify TB/non-TB infection, but also differentiate between human Mycobacterium tuberculosis and Mycobacterium bovis. The platform was verified in 37 clinical samples, statistically with 100% specificity and 95.2% sensitivity as compared to commercial real-time RPA. Overall, the proposed platform eliminates the need on external pumps and skilled personnel, holding promise to POC testing in the resource-limited area.

Keywords: Finger-driven; Microfluidics; Point-of-care; RPA; Tuberculosis.

MeSH terms

Biosensing Techniques*
Humans
Mycobacterium bovis*
Mycobacterium tuberculosis* / genetics
Point-of-Care Systems
Tuberculosis* / diagnosis