Products of two common alleles at the locus for human placental alkaline phosphatase differ by seven amino acids

Proc Natl Acad Sci U S A. 1986 Aug;83(15):5597-601. doi: 10.1073/pnas.83.15.5597.


Amino-terminal amino acid sequences (42 residues) were determined for the products of the three common alleles at the human placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC] gene locus. The sequences differ at position 3, which is proline in types 1 and 2 but is leucine in type 3. cDNA libraries were constructed in phage lambda gt11 and used to isolate clones covering the coding regions of types 1 and 3 cDNAs. Comparison of the deduced amino acid sequences of the types 1 and 3 proteins showed 7 differences out of 513 amino acids, each due to a single base substitution. cDNA sequence comparisons showed three silent substitutions in the coding regions and three base differences in the greater than 1 kilobase pairs of 3' untranslated sequences.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alkaline Phosphatase / genetics*
  • Alleles
  • Amino Acid Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Humans
  • Placenta / enzymology
  • Sequence Homology, Nucleic Acid


  • DNA
  • Alkaline Phosphatase

Associated data

  • GENBANK/M14169
  • GENBANK/M14170