Engineering Polysaccharide-Based Hydrogel Photonic Constructs: From Multiscale Detection to the Biofabrication of Living Optical Fibers

Adv Mater. 2021 Dec;33(52):e2105361. doi: 10.1002/adma.202105361. Epub 2021 Oct 7.

Abstract

Solid-state optics has been the pillar of modern digital age. Integrating soft hydrogel materials with micro/nanooptics could expand the horizons of photonics for bioengineering. Here, wet-spun multilayer hydrogel fibers are engineered through ionic-crosslinked natural polysaccharides that serve as multifunctional platforms. The resulting flexible hydrogel structure and reversible crosslinking provide tunable design properties such as adjustable refractive index and fusion splicing. Modulation of the optical readout via physical stimuli, including shape, compression, and multiple optical inputs/outputs is demonstrated. The unique permeability of the hydrogels is also combined with plasmonic nanoparticles for molecular detection of SARS-CoV-2 in fiber-coupled biomedical swabs. A tricoaxial 3D printing nozzle is then employed for the continuous fabrication of living optical fibers. Light interaction with living cells enables the quantification and digitalization of complex biological phenomena such as 3D cancer progression and drug susceptibility. These fibers pave the way for advances in biomaterial-based photonics and biosensing platforms.

Keywords: 3D cancer models; SARS-CoV-2; dynamic drug tests; hydrogel optical fibers; natural polysaccharides; plasmonics.

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Antineoplastic Agents / therapeutic use
  • Biocompatible Materials / chemistry
  • Biosensing Techniques
  • COVID-19 / diagnosis
  • COVID-19 / virology
  • Cell Culture Techniques, Three Dimensional
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Gold / chemistry
  • Humans
  • Hydrogels / chemistry*
  • Metal Nanoparticles / chemistry
  • Neoplasms / drug therapy
  • Neoplasms / pathology
  • Optical Fibers*
  • Optics and Photonics / methods*
  • Polysaccharides / chemistry*
  • Printing, Three-Dimensional
  • SARS-CoV-2 / isolation & purification

Substances

  • Antineoplastic Agents
  • Biocompatible Materials
  • Hydrogels
  • Polysaccharides
  • Gold