A Nonradioactive Method to Measure Hyaluronan Synthase Activity

Davide Vigetti; Evgenia Karousou; Manuela Viola; Alberto Passi

doi:10.1007/978-1-0716-1398-6_6

A Nonradioactive Method to Measure Hyaluronan Synthase Activity

Methods Mol Biol. 2022:2303:63-70. doi: 10.1007/978-1-0716-1398-6_6.

Authors

Davide Vigetti¹, Evgenia Karousou¹, Manuela Viola¹, Alberto Passi²

Affiliations

¹ Department of Surgical and Morphological Sciences, University of Insubria, Varese, Italy.
² Department of Surgical and Morphological Sciences, University of Insubria, Varese, Italy. alberto.passi@uninsubria.it.

PMID: 34626370
DOI: 10.1007/978-1-0716-1398-6_6

Abstract

Hyaluronan (HA) is a component of the extracellular matrix that is involved in many physiological and pathological processes. As HA modulates several functions (i.e., cell proliferation and migration, inflammation), its presence in the tissues can have positive or negative effects. HA synthases (HAS) are a family of three isoenzymes located on the plasma membrane that are responsible for the production of such polysaccharide and, therefore, their activity is critical to determine the accumulation of HA in tissues. Here, we describe a nonradioactive method to quantify the HAS enzymatic activity in crude cellular membrane preparation.

Keywords: Enzymatic activity; Glycosaminoglycan synthesis; Hyaluronan; Hyaluronan synthase; UDP-sugars.

MeSH terms

Cell Membrane
Extracellular Matrix
Glucuronosyltransferase / metabolism*
Hyaluronan Receptors
Hyaluronan Synthases
Hyaluronic Acid

Substances

Hyaluronan Receptors
Hyaluronic Acid
Glucuronosyltransferase
Hyaluronan Synthases