Protein sequencing by tandem mass spectrometry

Proc Natl Acad Sci U S A. 1986 Sep;83(17):6233-7. doi: 10.1073/pnas.83.17.6233.


Methodology for determining amino acid sequences of proteins by tandem mass spectrometry is described. The approach involves enzymatic and/or chemical degradation of the protein to a collection of peptides which are then fractionated by high-performance liquid chromatography. Each fraction, containing as many as 10-15 peptides, is then analyzed directly, without further purification, by a combination of liquid secondary-ion/collision-activated dissociation mass spectrometry on a multianalyzer instrument. Interpretation of collision-activated dissociation mass spectra is described, and results are presented from a study of soluble peptides produced by treatment of apolipoprotein B with cyanogen bromide and trypsin.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence*
  • Apolipoproteins B*
  • Chromatography, High Pressure Liquid
  • Humans
  • Mass Spectrometry / methods*
  • Peptide Fragments / analysis


  • Apolipoproteins B
  • Peptide Fragments