Objective: To investigate the relationship between hypoxia inducible factor 1 (HIF1α) and Wilms' tumor 1associating protein (WTAP) expression level in t(8;21) acute myeloid leukemia cells.
Methods: The t(8;21) acute myeloid leukemia cell lines, including SKNO-1 and Kasumi-1 were treated by Echinomycin for 24 h, RT-qPCR and Western blot were used to detect the expression levels of WTAP mRNA and the protein. The CoCl 2 was used to induce the hypoxia of the cells for 24 h, the expression levels of HIF1α, WTAP protein were detected by Western blot.
Results: The expression level of WTAP mRNA and the protein in the echinomycin treated group was significantly lower than those in the control group (P<0.01). The expression level of WTAP protein in the CoCl2 treated group was significantly higher than those in the control group (P<0.05).
Conclusion: The inhibition of HIF1-α could down-regulates the expression of WTAP, while the up-regulation of HIF1α could up-regulates the expression of WTAP, which shows that there is a positive correlation of HIF1α and WTAP expression. This result suggesting that HIF1α may be involves in the expression regulation of WTAP gene.
题目: t(8;21)急性髓系白血病细胞中HIF1α与WTAP表达关系研究.
目的: 探讨t(8;21)急性髓系白血病细胞中HIF1α与WTAP表达的关系。.
方法: 用HIF1α特异性抑制剂棘霉素（Echinomycin）处理t(8;21)阳性急性髓系白血病细胞系SKNO-1、Kasumi-1 24 h， 采用RT-qPCR及Western blot检测WTAP mRNA及蛋白的表达水平。经CoCl2诱导细胞缺氧24 h，采用Western blot检测HIF1α、WTAP蛋白的表达水平。.
结果: Echinomycin处理组WTAP mRNA及蛋白表达水平显著低于对照组（P<0.01），CoCl2处理组WTAP蛋白表达水平显著高于对照组（P<0.05）。.
结论: 抑制HIF1α可使WTAP的表达下调，而上调HIF1α则可使WTAP的表达上调，HIF1α与WTAP表达水平呈正相关，表明HIF1α可能参与了对WTAP的调控。.