The Zebrafish Embryo as a Model to Test Protective Effects of Food Antioxidant Compounds

Molecules. 2021 Sep 24;26(19):5786. doi: 10.3390/molecules26195786.

Abstract

The antioxidant activity of food compounds is one of the properties generating the most interest, due to its health benefits and correlation with the prevention of chronic disease. This activity is usually measured using in vitro assays, which cannot predict in vivo effects or mechanisms of action. The objective of this study was to evaluate the in vivo protective effects of six phenolic compounds (naringenin, apigenin, rutin, oleuropein, chlorogenic acid, and curcumin) and three carotenoids (lycopene B, β-carotene, and astaxanthin) naturally present in foods using a zebrafish embryo model. The zebrafish embryo was pretreated with each of the nine antioxidant compounds and then exposed to tert-butyl hydroperoxide (tBOOH), a known inducer of oxidative stress in zebrafish. Significant differences were determined by comparing the concentration-response of the tBOOH induced lethality and dysmorphogenesis against the pretreated embryos with the antioxidant compounds. A protective effect of each compound, except β-carotene, against oxidative-stress-induced lethality was found. Furthermore, apigenin, rutin, and curcumin also showed protective effects against dysmorphogenesis. On the other hand, β-carotene exhibited increased lethality and dysmorphogenesis compared to the tBOOH treatment alone.

Keywords: antioxidant effect; carotenoids; oxidative stress; polyphenols; zebrafish embryo.

MeSH terms

  • Animals
  • Antioxidants / administration & dosage*
  • Antioxidants / pharmacology
  • Apigenin / administration & dosage
  • Apigenin / pharmacology
  • Biological Factors / administration & dosage*
  • Biological Factors / pharmacology
  • Carotenoids / administration & dosage*
  • Carotenoids / pharmacology
  • Curcumin / administration & dosage
  • Curcumin / pharmacology
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Embryo, Nonmammalian / drug effects
  • Embryonic Development / drug effects
  • Flavanones / administration & dosage
  • Flavanones / pharmacology
  • Lycopene / administration & dosage
  • Lycopene / pharmacology
  • Oxidative Stress / drug effects
  • Polyphenols / administration & dosage*
  • Polyphenols / pharmacology
  • Xanthophylls / administration & dosage
  • Xanthophylls / pharmacology
  • Zebrafish / embryology*
  • beta Carotene / administration & dosage
  • beta Carotene / adverse effects
  • beta Carotene / pharmacology
  • tert-Butylhydroperoxide / adverse effects*

Substances

  • Antioxidants
  • Biological Factors
  • Flavanones
  • Polyphenols
  • Xanthophylls
  • beta Carotene
  • Carotenoids
  • Apigenin
  • astaxanthine
  • tert-Butylhydroperoxide
  • naringenin
  • Curcumin
  • Lycopene