Rac1 promotes kidney collecting duct integrity by limiting actomyosin activity

J Cell Biol. 2021 Nov 1;220(11):e202103080. doi: 10.1083/jcb.202103080. Epub 2021 Oct 14.


A polarized collecting duct (CD), formed from the branching ureteric bud (UB), is a prerequisite for an intact kidney. The small Rho GTPase Rac1 is critical for actin cytoskeletal regulation. We investigated the role of Rac1 in the kidney collecting system by selectively deleting it in mice at the initiation of UB development. The mice exhibited only a mild developmental phenotype; however, with aging, the CD developed a disruption of epithelial integrity and function. Despite intact integrin signaling, Rac1-null CD cells had profound adhesion and polarity abnormalities that were independent of the major downstream Rac1 effector, Pak1. These cells did however have a defect in the WAVE2-Arp2/3 actin nucleation and polymerization apparatus, resulting in actomyosin hyperactivity. The epithelial defects were reversible with direct myosin II inhibition. Furthermore, Rac1 controlled lateral membrane height and overall epithelial morphology by maintaining lateral F-actin and restricting actomyosin. Thus, Rac1 promotes CD epithelial integrity and morphology by restricting actomyosin via Arp2/3-dependent cytoskeletal branching.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Actin Cytoskeleton / metabolism
  • Actins / metabolism
  • Actomyosin / metabolism*
  • Animals
  • Cell Adhesion / physiology
  • Cell Polarity / physiology
  • Cells, Cultured
  • Cytoskeleton / metabolism
  • Epithelial Cells / metabolism
  • Kidney Tubules, Collecting / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Myosin Type II / metabolism
  • Neuropeptides / metabolism*
  • Signal Transduction / physiology
  • rac1 GTP-Binding Protein / metabolism*


  • Actins
  • Neuropeptides
  • Rac1 protein, mouse
  • Actomyosin
  • Myosin Type II
  • rac1 GTP-Binding Protein