Metabolism of prostaglandins D2 and F2 alpha in primary cultures of rat hepatocytes

Biochim Biophys Acta. 1986 Dec 5;879(3):330-8.

Abstract

3H-Labeled prostaglandins D2 and F2 alpha rapidly degraded to more-polar metabolites in primary cultured rat hepatocytes. The metabolites of prostaglandins D2 and F2 alpha accumulated in the culture medium. The metabolites extracted by ethyl acetate at pH 3 were purified by silicic acid column and thin-layer chromatography of silica gel, and were analysed by gas chromatography-mass spectrometry. The major metabolites from prostaglandin D2 were identified as dinor-prostaglandin D1 (7 alpha,13-dihydroxy-9-ketodinorprost-11-enoic acid) and tetranor-prostaglandin D1 (5 alpha,11- dihydroxy-7-ketotetranorprost-9-enoic acid). Those from prostaglandin F2 alpha were identified as dinor-prostaglandin F1 alpha (7 alpha,9 alpha,13-trihydroxydinorprost-11-enoic acid), tetranor-prostaglandin F1 alpha (5 alpha,7 alpha,11-trihydroxytetranorprost-9-enoic acid) and 9 alpha,11 alpha,15-trihydroxyprost-13-ene-1,20-dioic acid. These data indicate that prostaglandins D2 and F2 alpha mainly degraded by beta-oxidation, which is the same process as reported earlier for prostaglandins E1 and E2, and that prostaglandin F2 alpha was also subjected to omega-oxidation.

MeSH terms

  • Animals
  • Cells, Cultured
  • Chromatography, Gas
  • Chromatography, Thin Layer
  • Dinoprost
  • Kinetics
  • Liver / metabolism*
  • Male
  • Mass Spectrometry
  • Prostaglandin D2
  • Prostaglandins D / metabolism*
  • Prostaglandins F / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Tritium

Substances

  • Prostaglandins D
  • Prostaglandins F
  • Tritium
  • Dinoprost
  • Prostaglandin D2