2D MOF-Based Photoelectrochemical Aptasensor for SARS-CoV-2 Spike Glycoprotein Detection

ACS Appl Mater Interfaces. 2021 Oct 27;13(42):49754-49761. doi: 10.1021/acsami.1c17574. Epub 2021 Oct 17.


A reliable and sensitive detection approach for SARS-CoV 2 is essential for timely infection diagnosis and transmission prevention. Here, a two-dimensional (2D) metal-organic framework (MOF)-based photoelectrochemical (PEC) aptasensor with high sensitivity and stability for SARS-CoV 2 spike glycoprotein (S protein) detection was developed. The PEC aptasensor was constructed by a plasmon-enhanced photoactive material (namely, Au NPs/Yb-TCPP) with a specific DNA aptamer against S protein. The Au NPs/Yb-TCPP fabricated by in situ growth of Au NPs on the surface of 2D Yb-TCPP nanosheets showed a high electron-hole (e-h) separation efficiency due to the enhancement effect of plasmon, resulting in excellent photoelectric performance. The modified DNA aptamer on the surface of Au NPs/Yb-TCPP can bind with S protein with high selectivity, thus decreasing the photocurrent of the system due to the high steric hindrance and low conductivity of the S protein. The established PEC aptasensor demonstrated a highly sensitive detection for S protein with a linear response range of 0.5-8 μg/mL with a detection limit of 72 ng/mL. This work presented a promising way for the detection of SARS-CoV 2, which may conduce to the impetus of clinic diagnostics.

Keywords: SARS-CoV-2; aptasensor; metal−organic framework; photoelectrochemical; plasmon; spike glycoprotein.

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Base Sequence
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • COVID-19 / diagnosis
  • DNA / chemistry
  • Electrochemical Techniques / instrumentation
  • Electrochemical Techniques / methods
  • Electrodes
  • Gold / chemistry
  • Gold / radiation effects
  • Humans
  • Immobilized Nucleic Acids / chemistry
  • Light
  • Limit of Detection
  • Metal Nanoparticles / chemistry
  • Metal Nanoparticles / radiation effects
  • Metal-Organic Frameworks / chemistry*
  • Pharynx / virology
  • Photochemical Processes
  • Porphyrins / chemistry
  • SARS-CoV-2 / chemistry*
  • Spike Glycoprotein, Coronavirus / analysis*
  • Spike Glycoprotein, Coronavirus / chemistry
  • Ytterbium / chemistry


  • Aptamers, Nucleotide
  • Immobilized Nucleic Acids
  • Metal-Organic Frameworks
  • Porphyrins
  • Spike Glycoprotein, Coronavirus
  • spike protein, SARS-CoV-2
  • Gold
  • DNA
  • Ytterbium