Isolation of ocotillol/ocotillone from Fouquieria splendens (Ocote) using a batch reactor

Prep Biochem Biotechnol. 2022;52(5):540-548. doi: 10.1080/10826068.2021.1972425. Epub 2021 Oct 17.


Exploitation of the "ocotillo" tree (Fouquieria splendens Engelm) to produce ocotillol/ocotillone (o/o) in Northern Mexico hasn't been explored to this date. In the present work, isolation of ocotillol/ocotillone was performed through a batch reactor, which allowed the elimination of alcohol and phenols present in the ocote plant, yielding only resins and waxes from the ocote trunks. The ocotillol/ocotillone was extracted from the remaining resin on the ocote logs by solvent extraction in a batch reactor, to be crystallized after its extraction using a rotary evaporator. FTIR, and NMR analysis exhibited the characteristic ginsenoside bands, while the UV-vis spectrum of ocotillol/ocotillone depicted an absorption band belonging to the O-H bonds, indicating that the group is anchored to the ginsenoside structure and not due to a water signal. Lastly, the thermogravimetric analysis described a common behavior among other ginsenosides. Production of ocotillo/ocotillone has an energy consumption of 3624 kWh/gr. With a cost of $0.478 USD in laboratory equipment, which translates as a plausible sustainable production of ocotillol/ocotillone.

Keywords: Extraction; isolation; ocotillol/ocotillone; sustainable production.

MeSH terms

  • Ginsenosides* / chemistry


  • Ginsenosides
  • ocotillol