Basophils priming in patients with chronic spontaneous urticaria

Postepy Dermatol Alergol. 2021 Aug;38(4):608-610. doi: 10.5114/ada.2021.108910. Epub 2021 Sep 17.

Abstract

Introduction: Basophils are one of the main target cells in chronic spontaneous urticaria (CSU). If cells present higher susceptibility to production and degranulation of pro-inflammatory factors, priming may be associated with severity of symptoms and therapy ineffectiveness.

Aim: To evaluate the spontaneous state of increase in basophil activity and their priming profile in patients with CSU.

Material and methods: The study sample included 22 patients diagnosed with CSU and 20 healthy volunteers without either allergy symptoms or CSU. In this study, we evaluate the presence of CD63 and CD63+CD203c at basophils surface by flow cytometry test (basophil activation test - BAT).

Results: We found that the percentage of activated basophils was higher in patients with CSU than in the control group and this difference was statistically significant (p < 0.05).

Conclusions: Our results indicate a greater degree of basophils activation in patients with CSU in remission than in the control group; it might be useful for identification of patients with predominance of the autoimmune variant of CSU and typing patients responding (responders) and refractory (non-responders) to treatment with antihistamines.

Keywords: CD203c; CD63; basophil; chronic spontaneous urticaria.