Exploring a possible association between the occurrence of the SERPINE1-675 4G/5G (rs1799889) polymorphism and the increased risk of esophageal cancer in the Caucasian population

Anna Agnieszka Klimczak-Bitner; Jan Bitner; Komei Hiruta; Janusz Szemraj

doi:10.1016/j.bbrep.2021.101147

Exploring a possible association between the occurrence of the SERPINE1-675 4G/5G (rs1799889) polymorphism and the increased risk of esophageal cancer in the Caucasian population

Biochem Biophys Rep. 2021 Oct 5:28:101147. doi: 10.1016/j.bbrep.2021.101147. eCollection 2021 Dec.

Authors

Anna Agnieszka Klimczak-Bitner¹, Jan Bitner², Komei Hiruta³, Janusz Szemraj²

Affiliations

¹ Department of Biomedical Chemistry, Medical University of Lodz, 92-215, Lodz, Poland.
² Department of Medicinal Biochemistry, Medical University of Lodz, 92-215, Lodz, Poland.
³ Graduate School of Science and Technology, Keio University, Japan.

Abstract

The goal of this research was to analyze the SERPINE1 -675 4G/5G (rs1799889) and MMP9 T-1702A (rs2297864) polymorphisms in esophageal cancer among polish patients, classified as part of the Caucasian population. The analysis of polymorphic gene variants was performed on 35 randomly selected samples excised from patients with esophageal cancer. The tissue specimens were stored as Formalin-Fixed, Paraffin-Embedded (FFPE) blocks. All patients in the sample group were of Caucasian ethnicity. The genotype distribution of MMP9 T-1702A and SERPINE1 -675 polymorphisms was analyzed using the Restriction Fragment Length Polymorphism (RFLP) method. A correlation between the expression of -675 polymorphic form of SERPINE1 and alcohol abuse has been found. Additionally, a correlation between the -675 polymorphism and the subtype of EC developed by the patient has been shown. To the best of the authors' knowledge, this is the first report investigating the SERPINE1 -675 4G/5G (rs1799889) polymorphism as a potential candidate for a prognostic biomarker of esophageal cancer.

Keywords: Biomarker; MMP9 T1702A; Oesophgeal cancer; Polymorphism; SERPINE1 -675 4G/5G; rs1799889.