Identification of Candidate Olfactory Genes in Scolytus schevyrewi Based on Transcriptomic Analysis

Front Physiol. 2021 Oct 4;12:717698. doi: 10.3389/fphys.2021.717698. eCollection 2021.

Abstract

The bark beetle, Scolytus schevyrewi (S. schevyrewi), is an economically important pest in China that causes serious damage to the fruit industry, particularly, in Xinjiang Province. Chemical signals play an important role in the behavior of most insects, accordingly, ecofriendly traps can be used to monitor and control the target pests in agriculture. In order to lay a foundation for future research on chemical communication mechanisms at the molecular level, we generate antennal transcriptome databases for male and female S. schevyrewi using RNA sequencing (RNA-seq) analysis. By assembling and analyzing the adult male and female antennal transcriptomes, we identified 47 odorant receptors (ORs), 22 ionotropic receptors (IRs), 22 odorant-binding proteins (OBPs), and 11 chemosensory proteins (CSPs). Furthermore, expression levels of all the candidate OBPs and CSPs were validated in different tissues of male and female adults by semiquantitative reverse transcription PCR (RT-PCR). ScosOBP2 and ScosOBP18 were highly expressed in female antennae. ScosCSP2, ScosCSP3, and ScosCSP5 were specifically expressed in the antennae of both males and females. These results provide new potential molecular targets to inform and improve future management strategies of S. schevyrewi.

Keywords: Scolytus schevyrewi; antennae; expression analysis; olfactory genes; transcriptome.