How to Detect QTLs in the Plant Circadian Clock

Methods Mol Biol. 2022;2398:89-97. doi: 10.1007/978-1-0716-1912-4_8.


One of the most powerful methods to identify loci controlling complex quantitative traits has been the quantitative trait locus (QTL) mapping. The QTL mapping approach has proven immensely useful to improve our understanding of key pathways such as flowering time, growth, and disease resistance. Since major circadian clock parameters such as period, phase, and amplitude are quantitative in nature, the QTL mapping approach could also be used to study the complex genetic architecture of the circadian clock. Here, we describe a simple QTL mapping method to identify components controlling clock parameters in natural populations of Arabidopsis thaliana.

Keywords: Circadian clock; Linkage map; Luciferase assays; Natural variation; QTL mapping.

MeSH terms

  • Arabidopsis / genetics
  • Chromosome Mapping
  • Circadian Clocks* / genetics
  • Phenotype
  • Quantitative Trait Loci