Proteomic analysis of the host-pathogen interface in experimental cholera

Nat Chem Biol. 2021 Nov;17(11):1199-1208. doi: 10.1038/s41589-021-00894-4. Epub 2021 Oct 21.


The microbial cell surface is a site of critical microbe-host interactions that often control infection outcomes. Defining the set of host proteins present at this interface has been challenging. Here we used a surface-biotinylation approach coupled to quantitative mass spectrometry to identify and quantify both bacterial and host proteins present on the surface of diarrheal fluid-derived Vibrio cholerae in an infant rabbit model of cholera. The V. cholerae surface was coated with numerous host proteins, whose abundance were driven by the presence of cholera toxin, including the C-type lectin SP-D. Mice lacking SP-D had enhanced V. cholerae intestinal colonization, and SP-D production shaped both host and pathogen transcriptomes. Additional host proteins (AnxA1, LPO and ZAG) that bound V. cholerae were also found to recognize distinct taxa of the murine intestinal microbiota, suggesting that these host factors may play roles in intestinal homeostasis in addition to host defense.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / analysis*
  • Cholera / microbiology*
  • Host-Pathogen Interactions
  • Mice
  • Mice, Inbred C57BL
  • Proteomics*
  • Vibrio cholerae / chemistry*


  • Bacterial Proteins