Evaluation of the Physicochemical and Biological Properties of EndoSequence BC Sealer HiFlow

Akshita Mann; Yu Zeng; Timothy Kirkpatrick; Ransome van der Hoeven; Renato Silva; Ariadne Letra; Letícia Chaves de Souza

doi:10.1016/j.joen.2021.10.001

Evaluation of the Physicochemical and Biological Properties of EndoSequence BC Sealer HiFlow

J Endod. 2022 Jan;48(1):123-131. doi: 10.1016/j.joen.2021.10.001. Epub 2021 Oct 20.

Authors

Akshita Mann¹, Yu Zeng², Timothy Kirkpatrick¹, Ransome van der Hoeven³, Renato Silva⁴, Ariadne Letra⁵, Letícia Chaves de Souza⁶

Affiliations

¹ Department of Endodontics, University of Texas Health Science Center at Houston, School of Dentistry at Houston, Houston, Texas.
² Center for Craniofacial Research, University of Texas School of Dentistry at Houston, Houston, Texas.
³ Department of Diagnostic and Biomedical Sciences, University of Texas Health Science Center at Houston, School of Dentistry at Houston, Houston, Texas.
⁴ Department of Endodontics, University of Texas Health Science Center at Houston, School of Dentistry at Houston, Houston, Texas; Center for Craniofacial Research, University of Texas School of Dentistry at Houston, Houston, Texas.
⁵ Department of Endodontics, University of Texas Health Science Center at Houston, School of Dentistry at Houston, Houston, Texas; Center for Craniofacial Research, University of Texas School of Dentistry at Houston, Houston, Texas; Department of Diagnostic and Biomedical Sciences, University of Texas Health Science Center at Houston, School of Dentistry at Houston, Houston, Texas.
⁶ Department of Endodontics, University of Texas Health Science Center at Houston, School of Dentistry at Houston, Houston, Texas; Center for Craniofacial Research, University of Texas School of Dentistry at Houston, Houston, Texas. Electronic address: Leticia.ChavesdeSouza@uth.tmc.edu.

PMID: 34678358
DOI: 10.1016/j.joen.2021.10.001

Abstract

Introduction: Understanding the physicochemical and biological properties of endodontic sealers is important for endodontic treatment planning. This study evaluated the properties of EndoSequence BC Sealer HiFlow (BCH; Brasseler USA, Savannah, GA), EndoSequence BC Sealer (BC, Brasseler USA), and AH Plus (AHP; Dentsply DeTrey, Konstanz, Germany). The effect of temperature on the setting time and flow of these sealers was also evaluated.

Methods: The setting time, flow, radiopacity, pH, solubility, and calcium release were investigated following ISO guidelines. The morphology and chemical composition of the sealers were evaluated by scanning electron microscopy and energy-dispersive spectroscopy. The antibacterial activity of sealers was tested against 2 strains of Enterococcus faecalis. Sealer cytotoxicity and the effects on messenger RNA expression of proinflammatory and mineralization genes were also investigated. Data analysis was performed using analysis of variance, Tukey, Kruskal-Wallis, and Dunn multiple comparison tests. P ≤ .05 was considered statistically significant.

Results: The setting time and flow rate of all sealers were affected by heat (P ≤ .05). The setting times and solubility of BCH and BC were significantly higher than AHP (P ≤ .0001). The radiopacity of AHP was higher than BCH and BC (P ≤ .0001). All sealers were alkaline and had antibacterial effects. Cell viability was higher for BCH and BC than AHP (P ≤ .0001). No significant differences in messenger RNA expression of proinflammatory and mineralization genes were observed.

Conclusions: Overall, BCH and BC had similar physicochemical and biological properties. The observed high solubility of BCH and BC as well as the high cytotoxicity of AHP might negatively impact the clinical performance of these materials. The application of heat affected the setting time and flow of all sealers.

Keywords: Biological properties; EndoSequence BC sealer; HiFlow; endodontic sealer; physical properties; warm vertical compaction.

MeSH terms

Calcium Phosphates*
Drug Combinations
Germany
Humans
Materials Testing
Oxides*
Root Canal Filling Materials* / chemistry
Silicates*

Substances

Calcium Phosphates
Drug Combinations
EndoSequence root repair material
Oxides
Root Canal Filling Materials
Silicates