Reprogramming of a human induced pluripotent stem cell line from a long QT syndrome patient harboring a heterozygous mutation of c.1537C > T in SCN5A gene

Jianan Chen; Yesang Song; Junqing Gu; Lian Chen

doi:10.1016/j.scr.2021.102576

Reprogramming of a human induced pluripotent stem cell line from a long QT syndrome patient harboring a heterozygous mutation of c.1537C > T in SCN5A gene

Stem Cell Res. 2021 Dec:57:102576. doi: 10.1016/j.scr.2021.102576. Epub 2021 Oct 18.

Authors

Jianan Chen¹, Yesang Song¹, Junqing Gu¹, Lian Chen²

Affiliations

¹ Department of Cardiology, Yuyao People's Hospital of Zhejiang Province, Yuyao 315400, China.
² Department of Cardiology, Yuyao People's Hospital of Zhejiang Province, Yuyao 315400, China. Electronic address: yyrmyychenlian@163.com.

PMID: 34678660
DOI: 10.1016/j.scr.2021.102576

Abstract

Long QT syndrome (LQTS) often causes malignant arrhythmia and syncope. Mutations in the SCN5A gene are the main cause of type 3 LQTS. In this study, we generated a human induced pluripotent stem cell line ZJYYPHi001-A from a female LQTS patient harboring a heterozygous mutation in SCN5A using non-integrative Sendai virus. This cell line expressed pluripotency markers, had the normal karyotype and could differentiate into three germ layers in vitro.