Mesenchymal stem cells prevent ovariectomy-induced osteoporosis formation in mice through intraosseous vascular remodeling

Biochem Biophys Res Commun. 2021 Dec 10;582:64-71. doi: 10.1016/j.bbrc.2021.10.033. Epub 2021 Oct 19.

Abstract

Mesenchymal stem cells (MSCs) can promote osteogenesis and are a promising therapy for postmenopausal osteoporosis. However, the relationship between improved intraosseous microcirculation and increased bone mass induced by MSCs in postmenopausal osteoporosis remains unclear. After the primary MSCs were characterized, they were transplanted into ovariectomized mice. MSCs transplantation enhanced the trabecular number, trabecular bone volume/total volume, and trabecular bone mineral density in ovariectomized mice. To determine the role of MSCs in vascular repair, mice were subjected to femoral artery ligation. Through laser speckle flowmetry, vascular perfusion and femoral trabecular bone and cortical bone analyses, we determined the effects of MSCs in promoting intraosseous angiogenesis and preventing osteoporosis in mice. MSCs effectively prevented postmenopausal osteoporosis development, which is associated with the involvement of MSCs in reestablishment of microcirculation within the skeleton.

Keywords: Bone marrow mesenchymal stem cell; Bone mass recovery; Cellular therapy; Postmenopausal osteoporosis; Revascularization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Density
  • Disease Models, Animal
  • Female
  • Femoral Artery / pathology
  • Femoral Artery / surgery
  • Femur / blood supply
  • Femur / diagnostic imaging
  • Femur / pathology
  • Flow Cytometry
  • Humans
  • Ligation
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Microcirculation / physiology
  • Neovascularization, Physiologic*
  • Osteogenesis / physiology
  • Osteoporosis, Postmenopausal / diagnostic imaging
  • Osteoporosis, Postmenopausal / pathology
  • Osteoporosis, Postmenopausal / therapy*
  • Ovariectomy / methods*
  • Tomography, X-Ray Computed
  • Vascular Remodeling / physiology*