High expression of NFX1-123 in HPV positive head and neck squamous cell carcinomas

Head Neck. 2022 Jan;44(1):177-188. doi: 10.1002/hed.26906. Epub 2021 Oct 25.


Background: High-risk human papillomaviruses (HR HPV) cause nearly all cervical cancers and, in the United States, the majority of head and neck cancers (HNSCCs). NFX1-123 is overexpressed in cervical cancers, and NFX1-123 partners with the HR HPV type 16 E6 oncoprotein to affect multiple growth, differentiation, and immune response genes. However, neither the expression of NFX1-123 nor the levels of these genes have been investigated in HPV positive (HPV+) or negative (HPV-) HNSCCs.

Methods: The Cancer Genome Atlas Splicing Variants Database and HNSCC cell lines were used to quantify expression of NFX1-123 and cellular genes increased in cervical cancers.

Results: NFX1-123 was increased in HPV+ HNSCCs compared to HPV- HNSCCs. LCE1B, KRT16, SPRR2G, and FBN2 were highly expressed in HNSCCs compared to normal tissues. Notch1 and CCNB1IP1 had greater expression in HPV+ HNSCCs compared to HPV- HNSCCs.

Conclusion: NFX1-123 and a subset of its known targets were increased in HPV+ HNSCCs.

Keywords: NFX1-123; Notch1; head and neck squamous cell carcinoma; human papillomavirus; oropharyngeal cancer.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Head and Neck Neoplasms* / genetics
  • Humans
  • Papillomaviridae / genetics
  • Papillomavirus Infections* / genetics
  • Repressor Proteins / genetics
  • Squamous Cell Carcinoma of Head and Neck / genetics


  • NFX1 protein, human
  • Repressor Proteins