Effect of the Forth and Fifth Zinc Finger Deletions of MTF-1 on the Expression of Metal Ion Metabolism Related Gene

Dokl Biochem Biophys. 2021 Sep;500(1):385-392. doi: 10.1134/S1607672921050082. Epub 2021 Oct 25.

Abstract

Metal response element binding transcription factor 1 (MTF-1) is one of the important regulatory proteins involved in the mediation of intracellular metal ion balance, which is zinc dependent. The changes of zinc finger effected its function. MTF-1 mutant 293T cell line was obtained by transferring the vector of MTF-1 4th or 5th mutant zinc finger into 293T cell line that knocked out MTF-1 gene. The results showed that the mutant of 4th zinc finger in MTF-1 protein showed a significant difference on target gene expression compared with 5th zinc finger. Further RNA-seq assay showed that 4th and 5th zinc finger of MTF-1 have a different effect on molecular biological functions, cellular components, and biological process. The mutant of 4th and 5th zinc finger in MTF-1 protein changed different signaling pathways and metal ion metabolism related to genes. The present study evaluated that 4th or 5th mutant zinc finger in MTF-1 gene were associated with the function of MTF-1 protein.

Keywords: ERR signaling; MTF-1; metal ion metabolism; mutant; zinc finger.