Transforming growth factor-beta increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblasts

J Clin Invest. 1987 Apr;79(4):1285-8. doi: 10.1172/JCI112950.

Abstract

Transforming growth factor-beta (TGF beta), when injected subcutaneously into newborn mice, induces a rapid fibrotic response, stimulates chemotaxis, and elevates the rates of biosynthesis of collagen and fibronectin by fibroblasts in vitro. We explored the molecular mechanisms of TGF beta-mediated stimulation of collagen and fibronectin synthesis in cultured human foreskin fibroblasts. TGF beta preferentially stimulated the synthesis of fibronectin and type I procollagen chains 3-5-fold as shown by polypeptide analysis. Concomitant elevation in the steady state levels of messenger RNAs (mRNAs) coding for type I procollagen and fibronectin also occurred but without a net increase in the rate of transcription of either of these genes. The preferential stabilization of mRNAs specifying type I procollagen and fibronectin provides a partial explanation for the mechanisms by which TGF beta enhances the synthesis of type I procollagen and fibronectin in mesenchymal cells.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Collagen / biosynthesis
  • Electrophoresis, Polyacrylamide Gel
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Fibronectins / genetics*
  • Humans
  • Peptides / pharmacology*
  • Procollagen / genetics*
  • Protein Processing, Post-Translational*
  • RNA, Messenger / metabolism*
  • Skin / cytology
  • Skin / drug effects
  • Transforming Growth Factors

Substances

  • Fibronectins
  • Peptides
  • Procollagen
  • RNA, Messenger
  • Transforming Growth Factors
  • Collagen