Allulose Attenuated Age-Associated Sarcopenia via Regulating IGF-1 and Myostatin in Aged Mice

Mol Nutr Food Res. 2022 Jan;66(1):e2100549. doi: 10.1002/mnfr.202100549. Epub 2021 Nov 17.


Scope: Allulose is shown to increase the muscle weight in diet-induced obese mice. However, there are no studies on the effects of allulose in age-associated sarcopenia. This study aims to elucidate the mechanisms of action for allulose in age associated by analyzing the transcriptional patterns in aged mice.

Methods and results: The 48-week-old mice are fed with AIN-93diet containing allulose for 12 weeks. Allulose supplementation increases the muscle mass and grip strength in aged mice. Allulose increases the insulin-like growth factor 1 (IGF-1) and its downstream factor expressions which 40 are related protein synthesis, while inhibits the myostatin expression related protein degradation. In mRNA-seq analysis, allulose supplementation significantly decreases in Adiponectin, Adipsin, cell death inducing DFFA like effector (CIDEC), Haptoglobin, Neuroglobin, and stearoyl-CoA desaturase-1 (SCD1) and increases in cytokine-inducible SH2-containing protein (CISH) and ceramide synthase 1 (CerS1) that are regulate protein turn over in gastrocnemius. Also, allulose alleviates autophagy in muscle with regulated mammalian target of rapamycin (mTOR) signaling pathway and increases the anti-oxidant enzyme activity.

Conclusion: These findings suggest that allulose improves the age-associated sarcopenia with enhancing antioxidant properties by altering mRNA and protein expression.

Keywords: age-associated sarcopenia; aged mice; allulose; skeletal muscle function; skeletal muscle mass.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fructose
  • Insulin-Like Growth Factor I / genetics
  • Insulin-Like Growth Factor I / metabolism
  • Mammals
  • Mice
  • Mice, Obese
  • Muscle, Skeletal / metabolism
  • Myostatin / genetics
  • Myostatin / metabolism
  • Myostatin / pharmacology
  • Sarcopenia* / drug therapy
  • Sarcopenia* / metabolism
  • Sarcopenia* / prevention & control


  • Myostatin
  • psicose
  • Fructose
  • Insulin-Like Growth Factor I