Dual activity of Meloidogyne incognita-regulated Musa acuminata Pathogenesis-related-10 (MaPR-10) gene

Gene. 2022 Jan 30;809:146041. doi: 10.1016/j.gene.2021.146041. Epub 2021 Oct 26.


Plant immunity to pathogen infections is a dynamic response that involves multiple organelles and defence signalling systems such as hypersensitive response (HR) and systemic acquired resistance (SAR). The latter requires the function of Pathogenesis-related (PR) proteins, a common plant protein family with diverse roles in plant innate immunity. Our previous proteomics study showed that a PR gene (ITC1587_Bchr9_P26466_MUSBA) was differentially regulated during a compatible banana-M. incognita interaction, substantiating the isolation of this gene in the current study. Here, we successfully isolated and characterised Pathogenesis-related-10 (PR10) gene with β-1,3-glucanase and ribonuclease (RNase) activities from two Musa acuminata cultivars (denoted as MaPR10) namely Berangan and Grand Naine (ITC1256). We found that MaPR10 cloned sequences possess glycine-rich loop domain and shared conserved motifs specific to PR10 gene group, confirming its identity as a member of this group. Interestingly, we also found a catalytic domain sequence for glycoside hydrolase family 16 (EXDXXE), unique only to MaPR10 cloned sequences. Two peptide variants closely related to the reference sequence ITC1587_Bchr9_P26466_MUSBA namely MaPR10-BeB5 and MaPR10-GNA5 were overexpressed and purified to test for their functionality. Here, we confirmed that both protein variants possess β-1,3-glucanase and ribonuclease (RNase) activities, and inhibit the growth of Aspergillus fumigatus, a human opportunistic pathogen. To our knowledge, this is the first PR10 plant proteins with such properties to be reported thus far.

Keywords: Antifungal enzyme; Aspergillus fumigatus; Meloidogyne incognita; Musa spp.; Pathogenesis-related-10 gene; Ribonuclease; β-1,3-Glucanase.

MeSH terms

  • Animals
  • Antifungal Agents / pharmacology
  • Aspergillus fumigatus / drug effects
  • Aspergillus niger / drug effects
  • Gene Expression Regulation, Plant
  • Host-Parasite Interactions / genetics
  • Musa / genetics*
  • Musa / parasitology*
  • Onions / genetics
  • Phylogeny
  • Plant Immunity / genetics
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism*
  • Plant Proteins / pharmacology
  • Plant Roots / genetics
  • Plant Roots / parasitology
  • Plants, Genetically Modified
  • Tylenchoidea / pathogenicity*


  • Antifungal Agents
  • Plant Proteins