Wound Healing Effectivity of the Ethanolic Extracts of Ageratum conyzoides L. Leaf (White and Purple Flower Type) and Centella asiatica and Astaxanthin Combination Gel Preparation in Animal Model

Turk J Pharm Sci. 2021 Oct 28;18(5):609-615. doi: 10.4274/tjps.galenos.2021.34676.

Abstract

The study's objective was to determine the wound healing activity of the combination of ethanolic extracts of Ageratum conyzoides L. leaf (white and purple), Centella asiatica, and astaxanthin gel preparation.

Materials and methods: For in-gel preparation, three different formulas of gelling agents, namely carbopol 934 (1%), hydroxypropyl methylcellulose (HPMC) (9%), and natirum-carboxymethylcellulose (Na-CMC) (4%), were employed. Then, the organoleptic, pH, spreadability, and viscosity of the formulas were evaluated. To determine wound healing activity, six treatments, including negative control (placebo), positive control (bioplacenton), BP5 (A. conyzoides L. leaf ethanolic extract of white flower type 5%, C. asiatica L. Urb leaf ethanolic extract 2.5%, astaxanthin 0.05%), BU5 (A. conyzoides L. leaf ethanolic extract of purple flower type 5%, C. asiatica L. Urb leaf ethanolic extract 2.5%, astaxanthin 0.05%), BU10 (A. conyzoides L. leaf ethanolic extract of purple flower type 10%, C. asiatica L. Urb leaf ethanolic extract 5%, and astaxanthin 0.1%), and BP10 (A. conyzoides L. leaf ethanolic extract of white flower type 10%, C. asiatica L. Urb leaf ethanolic extract 5%, and astaxanthin 0.1%) were evaluated. All treatments were applied to an incision wound (1.5 cm). Measurement of the wound length was conducted daily for 14 days.

Results: The results showed that the carbopol 934 (1%) gelling agent formula was better than HPMC and Na-CMC. Meanwhile, the percentages of wound healing activity for negative, positive, BP5, BU5, BU10, and BP10 groups were 72.51%, 69.36%, 70.14%, 81.70%, 86.54%, and 80.21%, respectively. The BU5 and BU10 showed significant activity (p<0.05) compared with positive and negative controls.

Conclusion: BU10 provided the best wound healing activity and can be developed as a commercial product.

Keywords: Ageratum conyzoides L.; Centella asiatica; astaxanthin; gel preparation; wound healing.