New Type III CRISPR variant and programmable RNA targeting tool: Oh, thank heaven for Cas7-11

Ryan J Catchpole; Michael P Terns

doi:10.1016/j.molcel.2021.10.014

New Type III CRISPR variant and programmable RNA targeting tool: Oh, thank heaven for Cas7-11

Mol Cell. 2021 Nov 4;81(21):4354-4356. doi: 10.1016/j.molcel.2021.10.014.

Authors

Ryan J Catchpole¹, Michael P Terns²

Affiliations

¹ Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
² Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA; Department of Genetics, University of Georgia, Athens, GA 30602, USA; Department of Microbiology, University of Georgia, Athens, GA 30602, USA. Electronic address: mterns@uga.edu.

Abstract

Özcan et al. (2021) and van Beljouw et al. (2021) characterize a novel Type III-E CRISPR-Cas subtype, composed of a single polypeptide with crRNA processing and sequence-specific RNA cleavage activities, that provides a new RNA knockdown tool for mammalian cells with fewer off-target effects than current technologies.

Publication types

Research Support, N.I.H., Extramural
Comment

MeSH terms

CRISPR-Associated Proteins* / genetics
RNA / genetics
RNA Cleavage

Substances

CRISPR-Associated Proteins
RNA

Grants and funding

R35 GM118160/GM/NIGMS NIH HHS/United States