A simple and ultrasensitive metal-organic framework-based aptasensor for fluorescence detection of ethanolamine

Abstract

In this research, a facile fluorescence aptasensor was designed for the ultrasensitive determination of ethanolamine (ETA) as an aliphatic amino alcohol molecule with harmful side effects for human health. Zeolitic imidazolate framework-8 (ZIF-8) as an efficient metal-organic framework was applied to quench the fluorescence emission of the FAM-labeled ETA aptamer. The presence of ETA could recover the fluorescence response under the optimal experimental conditions. ETA could be sensitively detected by the designed aptasensor in the linear concentration range of 0.1 nM-20 µM with the detection limit of 14.38 pM. The designed aptasensor was utilized to determine ETA in milk and serum samples with the detection limits of 17.86 and 15.21 pM, respectively. The aptasensor with the remarkable features of high sensitivity, simplicity and feasibility holds the supreme potential to construct a low-cost portable sensing method for food safety control, human diagnostics, and environmental monitoring with coupling to the microfluidic paper-based devices.

Keywords: Aptasensor; Ethanolamine; Florescence quenching; Metal-organic framework.