Comparison of different extraction methods of Brazilian "pacová" (Renealmia petasites Gagnep.) oilseeds for the determination of lipid and terpene composition, antioxidant capacity, and inhibitory effect on neurodegenerative enzymes

Luana Cristina Dos Santos; Gerardo Álvarez-Rivera; José David Sánchez-Martínez; Julio César Flores Johner; Francisco Manuel Barrales; Alessandra Lopes de Oliveira; Alejandro Cifuentes; Elena Ibáñez; Julian Martínez

doi:10.1016/j.fochx.2021.100140

Comparison of different extraction methods of Brazilian "pacová" (Renealmia petasites Gagnep.) oilseeds for the determination of lipid and terpene composition, antioxidant capacity, and inhibitory effect on neurodegenerative enzymes

Food Chem X. 2021 Oct 15:12:100140. doi: 10.1016/j.fochx.2021.100140. eCollection 2021 Dec 30.

Authors

Luana Cristina Dos Santos^{1

2}, Gerardo Álvarez-Rivera², José David Sánchez-Martínez², Julio César Flores Johner¹, Francisco Manuel Barrales¹, Alessandra Lopes de Oliveira³, Alejandro Cifuentes², Elena Ibáñez², Julian Martínez¹

Affiliations

¹ Laboratory of High Pressure in Food Engineering (LAPEA), Department of Food Engineering and Technology, School of Food Engineering, University of Campinas, R. Monteiro Lobato 80, 13083-862 Campinas, SP, Brazil.
² Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC, Nicolás Cabrera 9, 28049 Madrid, Spain.
³ Natural Products and High Pressure Technology Laboratory (LTAPPN), Department of Food Engineering, Faculty of Animal Science and Food Engineering, University of São Paulo (USP), 13635-900 Pirassununga, SP, Brazil.

Abstract

Pacová (Renealmia petasites Gagnep.) is a Brazilian native plant, usually cultivated in south regions of the country. Pacová was previously reported concerning their possible health benefits, mostly from folk medicine. However, only few works relates the health benefits with the composition of the fruit parts. In this context, this work aimed to bring, for the first time in literature, the chemical characterization in respect to lipid and terpene composition of R. petasites oilseed, performed by three different extraction methods (supercritical fluid extraction (SFE) with CO₂, Soxhlet with petroleum ether (SOX), and maceration with hexane (MAC)). SFE was most selective for MUFAs, PUFAs, sesqui- and diterpenes. The main terpene identified in all extracts was 2-carene. The extracts presented poor AChE inhibition, and SOX presented potential inhibitory effect against lipoxygenase activity. Overall, R. petasites oilseed is a natural source of terpenes and their potential health benefits are highly encouraged to be investigated.

Keywords: Renealmia petasites; antiinflammatory; antioxidant; supercritical CO2; terpenes.