Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line

Somat Cell Mol Genet. 1987 May;13(3):235-44. doi: 10.1007/BF01535205.


We are exploring the origin and function of amplified DNA sequences associated with double minutes (DMs) in a spontaneously transformed derivative of mouse 3T3 cells. Toward that goal, we have constructed a cDNA library using RNA from these cells and have isolated cDNA clones representing sequences that are amplified and overexpressed in these 3T3-DM cells. From results of Northern- and Southern-blot analyses, we conclude that these cDNAs represent two distinct genes, which we have designated mdm-1 and mdm-2. Using DNAs from a panel of Chinese hamster-mouse somatic cell hybrids together with in situ hybridization protocols for gene mapping studies, we have found that these DM-associated, amplified DNA sequences originate from mouse chromosome 10, region C1-C3. Sequences homologous to mdm-1 and mdm-2 are present in the genomes of several species examined, including that of man.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Cell Transformation, Neoplastic / genetics*
  • Chromosome Mapping
  • Cricetinae
  • Cricetulus
  • DNA / analysis
  • Fibroblasts / pathology
  • Gene Amplification*
  • Humans
  • Hybrid Cells / analysis
  • Mice
  • Mice, Inbred BALB C / genetics
  • Phylogeny
  • RNA, Messenger / genetics
  • Sequence Homology, Nucleic Acid
  • Species Specificity


  • RNA, Messenger
  • DNA