Co-immunofluorescence of MRPL12 and Nrf2 in HK2 Cells

Yao Lu; Xia Gu; Qiang Wan; Hong Feng; Yi Liu

doi:10.21769/BioProtoc.4191

Co-immunofluorescence of MRPL12 and Nrf2 in HK2 Cells

Bio Protoc. 2021 Oct 20;11(20):e4191. doi: 10.21769/BioProtoc.4191.

Authors

Yao Lu¹, Xia Gu¹, Qiang Wan², Hong Feng³, Yi Liu⁴

Affiliations

¹ School of Medicine, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China.
² Translational Research Center, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.
³ Cancer Center, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.
⁴ Department of Respiratory Medicine, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.

Abstract

Immunofluorescence is a technique to visualize the localization of specific molecule targets within cells using the specificity of antibodies. Here, we describe a protocol to detect two different protein components in a cell simultaneously. Antibody concentrations to be used vary from cell to cell and should be optimized for different cell types. In this protocol, we perform co-immunofluorescence of mitochondrial ribosomal protein L7/L12 (MRPL12) and nuclear factor erythroid 2-related factor 2 (Nrf2), a potential transcription factor of MRPL12, in HK-2 cells, as an example. Taking advantage of the diverse set of antibodies raised in different species, we are able to analyze the colocalization and expression of these proteins.

Keywords: Co-immunofluorescence staining; Colocation; HK-2 cells; MRPL12; Nrf2; Semi-quantitative analysis.