Degradation kinetics and isomerization of 5- O-caffeoylquinic acid under ultrasound: Influence of epigallocatechin gallate and vitamin C

Danli Wang; Jingjing Wang; Jiachen Sun; Shaoping Qiu; Bingquan Chu; Ruosi Fang; Ling Li; Jinyan Gong; Fuping Zheng

doi:10.1016/j.fochx.2021.100147

Degradation kinetics and isomerization of 5- O-caffeoylquinic acid under ultrasound: Influence of epigallocatechin gallate and vitamin C

Food Chem X. 2021 Oct 26:12:100147. doi: 10.1016/j.fochx.2021.100147. eCollection 2021 Dec 30.

Authors

Danli Wang^{1

2}, Jingjing Wang², Jiachen Sun², Shaoping Qiu², Bingquan Chu², Ruosi Fang², Ling Li², Jinyan Gong^{1

2}, Fuping Zheng¹

Affiliations

¹ Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China.
² Zhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, China.

Abstract

5-O-Caffeoylquinic acid (5-CQA), also known as chlorogenic acid, exhibits various biological activities. Hence the interest in its property change during processing and extraction has increased. The present work studied the influence of ultrasound on the stability of 5-CQA at different pH (pH 4.69, 7.09, 7.69 and 9.22) in water and 50% methanol-water system. Different parameters including solvent, ultrasonic power, time, temperature, duty cycle, and liquid height were investigated during the treatment. Results indicate that ultrasound accelerated the degradation of 5-CQA. Based on Weibull model, the degradation kinetics were described. The rate constant (k) of the degradation increased with the increasing pH, demonstrating the alkali sensitivity of 5-CQA. The isomerization of 5-CQA to 3- and 4-O-caffeoylquinic acid was found at neutral and alkaline conditions, which was further boosted by ultrasound. The stability of 5-CQA was improved by adding epigallocatechin gallate (EGCG) and vitamin C (VC) respectively.

Keywords: Ascorbic acid; Chlorogenic acid; Degradation kinetics; Epigallocatechin gallate; Isomerization; Ultrasound.