Fibronectin Molecular Status in Plasma of Women with Endometriosis and Fertility Disorders

Int J Mol Sci. 2021 Oct 22;22(21):11410. doi: 10.3390/ijms222111410.


The diagnosis of endometriosis and fertility disorders is difficult; therefore, it is necessary to look for reliable biomarkers. Analysis of the molecular status of fibronectin as a key player in repair and wound healing processes, as well as in coagulation and fibrinolysis pathways, is justified. ELISA and SDS-agarose immunoblotting were applied to determine the fibronectin concentration and presence and occurrence of soluble FN-fibrin complexes in the blood plasma of women with endometriosis (n = 38), fertility disorders (n = 28) and the healthy group (n = 25). The concentration of fibronectin in the blood plasma of women with endometriosis (292.61 ± 96.17 mg/L) and fertility disorders (287.53 ± 122.68 mg/L) was significantly higher than in the normal group (226.55 ± 91.98 mg/L). The presence of FN-fibrin complexes of 750, 1000, 1300, 1600 and 1900 kDa in the plasma of women with endometriosis and fertility disorders was shown. The presence of FN-fibrin complexes with a molecular mass of more than 1300 kDa in women with endometriosis and infertility and the complete absence of these complexes in healthy women may indicate an increased and chronic activation of coagulation mechanisms in these patients. The presence of complexes of high molecular mass may be one of the biomarkers of fertility disorders in women.

Keywords: endometriosis; fertility disorders; fibrin; molecular status of fibronectin.

MeSH terms

  • Adult
  • Biomarkers
  • Endometriosis / diagnosis
  • Endometriosis / metabolism*
  • Endometriosis / physiopathology
  • Female
  • Fibrin / metabolism
  • Fibrin Fibrinogen Degradation Products / analysis
  • Fibronectins / analysis
  • Fibronectins / blood
  • Fibronectins / metabolism*
  • Fibronectins / physiology
  • Humans
  • Infertility, Female / metabolism*
  • Infertility, Female / physiopathology
  • Middle Aged
  • Plasma / chemistry


  • Biomarkers
  • FN1 protein, human
  • Fibrin Fibrinogen Degradation Products
  • Fibronectins
  • Fibrin