Infection of Brain Pericytes Underlying Neuropathology of COVID-19 Patients

Int J Mol Sci. 2021 Oct 27;22(21):11622. doi: 10.3390/ijms222111622.


A wide range of neurological manifestations have been associated with the development of COVID-19 following SARS-CoV-2 infection. However, the etiology of the neurological symptomatology is still largely unexplored. Here, we used state-of-the-art multiplexed immunostaining of human brains (n = 6 COVID-19, median age = 69.5 years; n = 7 control, median age = 68 years) and demonstrated that expression of the SARS-CoV-2 receptor ACE2 is restricted to a subset of neurovascular pericytes. Strikingly, neurological symptoms were exclusive to, and ubiquitous in, patients that exhibited moderate to high ACE2 expression in perivascular cells. Viral dsRNA was identified in the vascular wall and paralleled by perivascular inflammation, as signified by T cell and macrophage infiltration. Furthermore, fibrinogen leakage indicated compromised integrity of the blood-brain barrier. Notably, cerebrospinal fluid from additional 16 individuals (n = 8 COVID-19, median age = 67 years; n = 8 control, median age = 69.5 years) exhibited significantly lower levels of the pericyte marker PDGFRβ in SARS-CoV-2-infected cases, indicative of disrupted pericyte homeostasis. We conclude that pericyte infection by SARS-CoV-2 underlies virus entry into the privileged central nervous system space, as well as neurological symptomatology due to perivascular inflammation and a locally compromised blood-brain barrier.

Keywords: ACE2; COVID-19; SARS-CoV-2; blood–brain barrier; brain; infection; multiplexed IHC; pericytes; vasculature.

MeSH terms

  • Angiotensin-Converting Enzyme 2 / genetics
  • Angiotensin-Converting Enzyme 2 / metabolism*
  • Animals
  • Blood-Brain Barrier
  • Brain / pathology
  • Brain / virology*
  • COVID-19 / etiology
  • COVID-19 / physiopathology*
  • Case-Control Studies
  • Encephalitis, Viral / pathology
  • Encephalitis, Viral / virology*
  • Fibrinogen / metabolism
  • Humans
  • Immunohistochemistry / methods
  • Mice
  • Pericytes / metabolism
  • Pericytes / pathology
  • Pericytes / virology*
  • Receptor, Platelet-Derived Growth Factor beta / cerebrospinal fluid


  • Fibrinogen
  • Receptor, Platelet-Derived Growth Factor beta
  • ACE2 protein, human
  • Ace2 protein, mouse
  • Angiotensin-Converting Enzyme 2